Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/reperfusion stress

Increasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it’s obscure how stress disturbs normal physiological process, then endogenous reactive species mend cases. Hypoxia/reperfusion (H/R), a common intractable injury in aquaculture clinic, can induce oxidative ultimately cause irreversible to organism. Cysteine (Cys) plays essential roles maintaining transduction numerous redox homeostasis subcellular structures, cells organisms. A great deal fluorescence research about Cys focusing on selective probes but poor exploration biofunction under stress. Therefore, it is significance examine bio-effects against H/R In present work, we design fluorescent probe BCy-AC for situ detecting Cys, unique Enol-Keto tautomerization fluorophore BCy-Keto propels reaction process which will improve sensitivity potential application performance probe. conveniently applied visualize HT-22 cells, zebrafish mice tissues. Moreover, imaging results obtained from models reveal that changes hypoxia reperfusion time pretreatment effectively defend vivo.